GMP-CKS-HE007-50μg / 询价
GMP-CKS-HE007-50μgx10 / 询价
GMP-CKS-HE007-50μgx20 / 询价
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GMP Human IL-7
Recombinant GMP Human IL-7 is expressed from E.coli without tag.It contains Asp26-His177 [Accession | P13232].
The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
≥ 95% as determined by SEC-HPLC.
≤ 0.01 EU per μg by the LAL method.
（Residual Host Cell Protein）
（Residual Host Cell DNA）
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival.
-20°C±5°C for 36 months in lyophilized state.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
GMP Human IL-7 on Bis-Tris PAGE under reduced condition.
Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of GMP Human IL-7 is > 1 × 10^8 IU/mg, which is calibrated against the human IL-7 WHO reference standard (NIBSC code: 90/530) (QC Test).
KACTUS GMP grade cytokines are manufactured and tested in accordance with relevant GMP guidelines and regulatory requirements, and are in compliance with ISO 13485:2016 certified quality system.
● Personnel training programs
● Clean rooms and facility
● Facility maintenance and equipment calibration & verification
● Raw material testing and supplier evaluation and approval
● Animal-free manufacturing
● Rigorous quality control
● Stability testing(freeze-thaw and accelerated stability of reconstituted protein; long-term stability in lyophilized state)
● Comprehensive support files, including COA, COO, MSDS, TSE/BSE statement, etc.
We conduct comprehensive quality control testing to ensure that KACTUS GMP grade cytokines meet strict standards for identification, purity, safety, bioactivity and batch-to-batch consistency.
● Identification by Bis-Tris PAGE
● Purity by SEC-HPLC
● Endotoxin level
● Residual host cell protein
● Residual host cell DNA
● Mycoplasma testing
● Sterility testing
● Bioactivity (Specific activity against standards of WHO if applicable)
● Batch-to-batch consistency
Interleukin (IL)-7 is a non-redundant cytokine in T-cell development and function. It is required in early T-cell development as well as for T-cell homeostasis and is secreted by stromal cells in the thymic and bone marrow (BM) environment. IL-7 binds to its receptor which is composed of the two chains IL-7Rα (CD127), shared with the thymic stromal lymphopoietin (TSLP), and the common γ chain (CD132) for IL-2, IL-15, IL-9 and IL-21. Whereas γc is expressed by most hematopoietic cells, IL-7Rα is nearly exclusively expressed on lymphoid cells. After binding to its receptor, IL-7 signals through two different pathways: Jak-Stat (Janus kinase-Signal transducer and activator of transcription) and PI3K/Akt responsible for differentiation and survival, respectively.
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
ElKassar N, Gress RE. An overview of IL-7 biology and its use in immunotherapy. J Immunotoxicol. 2010;7(1):1-7. doi:10.3109/15476910903453296
KACTUS GMP Human IL-7 is for research or ex vivo cell processing use, not for human or animal in vivo application.
